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To do this, the inventors made a large plasmid (˜12 kb) exchanging the p15a-based origin of replication by the primase origin. This plasmid targets the lacZ gene (p1322, SEQ ID NO: twenty five) and likewise incorporates a chloramphenicol marker. as it was disregarded if the RBS toughness would wish

Destined for the luxurious constitution industry, it has 5 large ensuite cabins, a jacuzzi within the foredeck and a completely air-conditioned flybridge with inner and exterior entry. in reality the HH 88 is designed to have not more than 4 degrees of heel to keep up the envisioned comfort and eas